TABLE 1.
Correlation of transcriptional activity of hybrid LTR At, replication of ACE-GFP-At, and presence of androgen receptor in human prostate carcinoma cell lines
| Cell line | Androgen receptor expression | Efficient transactivation of hybrid LTR Ata | Replication of ACE-GFP-Atb |
|---|---|---|---|
| LNCaP | + | + | + |
| MDA PCa 2b | + | + | + |
| DU145 | − | − | − |
| PC-3 | − | − | − |
| PC-3(AR)2 | + | + | + |
a
Cell lines denoted as positive exhibited luciferase expression levels 200-fold or more higher than those of promoterless control plasmids; cell lines denoted as negative exhibited levels less than 10-fold higher than those of promoterless controls. Data used were taken from transfections in charcoal-stripped serum containing 1 nM DHT.
b
Determined by flow cytometric analysis of infected cells over a 21-day period following exposure to stock vector.