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. 2005 Jun 24;89(3):1710–1720. doi: 10.1529/biophysj.104.056093

FIGURE 2.

FIGURE 2

Carboxy-terminal truncations modify the voltage dependence of fast and slow gating. (A) Voltage dependence of the open probability. (B) Voltage dependence of the fast (solid symbols) and slow (open symbols) deactivation time constants for WT (•), R894X (▾), and K875X (▪) hClC-1 channels. Mean ± SE (n > 58). The voltage dependence of the fast deactivation time constants was fitted with τ = 1/(αoeaeoV/kT + βoe−bzoV/kT), giving an equivalent gating charge of 1 for the opening rate constant and 0.75 for the closing time constants. Slow time constants were fitted with single exponentials. (C and D) Voltage dependence of the slow (C) and the fast (D) gate of WT (•) and mutant (R894X, ▾, and K875X, ▪) channels. (E and F) Plot of the midpoint of the voltage dependence of slow (E) and fast (F) gating versus [Cl−]o. Mean ± SE (n > 8).