TABLE 1.

Gating properties of WT and truncated ClC-1 channels

	Popen under standard conditions V0.5 (mV)	Fast gate
		V0.5 fast under standard conditions (mV)	Pmin fast under standard conditions	α1 fast(0) (s−1)	z (α1 fast)	γfast(0) (s−1)	I (γfast)	KC fast(Cl−) at −35 mV (mM)
hClC-1	−75.9 ± 1.7	−90.5 ± 0.8	0.18 ± 0.03	0.69	−0.61	677.9	0.42	54.1 ± 3.1
	n = 79	n = 13	n = 13					n = 9
R894X hClC-1	−96.2 ± 0.8	−107.8 ± 1.8	0.23 ± 0.02	0.42	−0.55	1088.7	0.50	33.0 ± 1.5
	n = 58	n = 8	n = 8					n = 10
	p < 0.01	p < 0.01	p = 0.51					p < 0.01
K875X hClC-1	−21.4 ± 1.1	−59.0 ± 1.5	0.27 ± 0.05	0.52	−0.47	258.4	0.33	155.0 ± 20.5
	n = 71	n = 13	n = 13					n = 5
	p < 0.01	p < 0.01	p = 0.25					p < 0.01
		Slow gate
		V0.5 slow under standard conditions (mV)	Pmin slow under standard conditions	α1 slow(0) (s−1)	z (α1 slow)	γslow(0) (s−1)	z (γslow)	KC slow(Cl−) at −35 mV (mM)
hClC-1		−74.7 ± 2.2	0.54 ± 0.02	0.46	−0.61	16.43	0.54	16.7 ± 0.8
		n = 14	n = 14					n = 6
R894X hClC-1		−98.4 ± 2.0	0.77 ± 0.08	n.d.	n.d.	n.d.	n.d.	n.d.
		n = 8	n = 8
		p < 0.01	p < 0.01
K875X hClC-1		−25.5 ± 1.8	0.23 ± 0.01	0.55	−0.60	13.08	0.51	45.3 ± 6.7
		n = 13	n = 13					n = 7
		p < 0.01	p < 0.01					P < 0.01

n.d.: not determined.