FIG. 4.

HCV RNA accumulation after transfection of Huh-7.5 cells with full-length HCV RNA. One microgram of in vitro-transcribed RNA was electroporated into Huh-7.5, and 2 × 10⁵ cells were plated into 35-mm-diameter wells. Total cellular RNA was isolated at 24, 48, and 96 h posttransfection, and HCV RNA levels were quantified as described in the Materials and Methods. The ratio of HCV RNA relative to the pol⁻ defective subgenomic RNA (HCV RNA/pol⁻) was plotted against the time posttransfection, and similar results were obtained when this experiment was repeated