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. 2005 Jun 24;89(3):1833–1840. doi: 10.1529/biophysj.105.061812

FIGURE 1.

FIGURE 1

Effect of rhodopsin/18:0,22:6n3PC ratios on DPH rotational dynamics. Time-resolved DPH fluorescence anisotropy was measured at 37°C in pH 7 PBS buffer. (A) The anisotropy decays were analyzed using an empirical sum-of-exponential model. The resulting rotational correlation times were averaged to obtain 〈φ〉. (B) The anisotropy decays were analyzed using the BRD model as described in the Materials and Methods section. D⊥ is the rotational diffusional coefficient of DPH obtained from the BRD analysis. Rhodopsin/lipid ratios are expressed as molar ratios.