Bilayer orientational order as a function of the mole fraction of DMPC. Sbilayer was calculated from the 31P NMR chemical shift as per Eq. 1 in the text. The mole fraction of DMPC was calculated using XDMPC = q/(1+q) where q was derived from the relative integrated intensities of the 31P NMR resonances of DMPC versus DHPC. Circles, DMPC; triangles, DHPC. Open symbols, 25 wt % lipid; solid symbols, 15 wt % lipid. The straight lines show linear regression fits to the combined 15 and 25 wt % lipid data. For DMPC the regression line has slope 3.13, intercept −1.73, and r2 0.87. For DHPC the regression line has slope 1.81, intercept −1.14, and r2 0.86.