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. 2002 Dec;76(24):13015–13027. doi: 10.1128/JVI.76.24.13015-13027.2002

FIG. 3.

FIG. 3.

Western analysis of Rep gene induction by Ad-Cre infection. rep gene induction by Ad-Cre infection either in plasmid transfection experiments (lanes 1 to 4) or in cell lines (lanes 5 to 8). Plasmids pXX2-Int-A3-Hyg-2 (lanes 1 and 2) and plasmid pXX2-Int-A3-Hyg-8 (lanes 3 and 4) had no rep gene expression in transfection (lanes 1 and 3) but showed high-level Rep gene expression at 48 h after Ad-Cre infection (lanes 2 and 4). Lanes 5 to 8 are examples of Cre-inducible 293 cell-based AAV packaging cell lines. After Ad-Cre infection for 48 h, two clones showed very high rep gene expression (lanes 5 and 7), and two clones showed modest rep gene expression (lanes 6 and 8).