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Acrylamide quenching of F344W-MDE under apo (○), ADP (♦), and ATP (□) conditions. F0/F was plotted against [acrylamide] and fit to the Stern-Volmer equation (Eq. 2). The resulting Stern-Volmer constants were divided by the fluorescence lifetime under each experimental condition in the absence of acrylamide to determine the appropriate bimolecular quenching constants (kq).
