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The critical spermidine concentration at the abrupt change in interaxial spacing is strongly dependent on the nature of the anion. Dint is shown as a function of spermidine concentration for the salt SpdCl3 (•) and for spermidine base neutralized with stoichiometrically added acid, H2SO4 (▪), HCl (▾), and CH3CO2H (♦). Interaxial spacings were determined as described in Fig. 2. The final pH for the acid-neutralized samples was ∼6.8.
