TABLE 1.

Parameters describing the nonelectrostatic binding of gp32 and its mutants to single-stranded nucleic acids and to the gp32 C-terminal domain

		log(K0ω)	−log(K0CTDNCTD)	ΔG0CTD kcal/mol
gp32/ss λ-DNA	7.90	6.50 ± 0.20	1.4 ± 0.2	1.93 ± 0.20
gp32/poly(A)	7.15	5.75 ± 0.20	1.4 ± 0.2	1.93 ± 0.20
K3A/poly(A)	6.72	5.02 ± 0.20	1.7 ± 0.2	2.40 ± 0.20
R4T/poly(A)	6.10	4.10 ± 0.20	2.0 ± 0.2	2.60 ± 0.20

In Table 1, we have used Eq. 5: The value ΔG_0CTD was calculated as ΔG_0CTD = −k_BT · ln(K_0CTD · N_CTD), and we took into account that at room temperature (T_r), k_BT_r = 0.59 kcal/mol.