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. 2002 Dec;76(24):12981–12991. doi: 10.1128/JVI.76.24.12981-12991.2002

FIG. 7.

FIG. 7.

Suppression of RNA silencing by the γb proteins of PSLV and BSMV. (A to D) Effect of the hordeivirus γb proteins on the symptoms of PVX infection. (A) Noninfected control plant of N. benthamiana. (B) Symptoms of PVX infection. (C and D) Necrosis in the systemically infected leaves in plants inoculated with PVX-PSγb and PVX-BSγb, respectively. (E to H) GFP expression from chimeric TMV (TMV-GFP) in the cross-protection assay. Pictures were taken under long-wave UV illumination. (E) GFP fluorescence in the inoculated and systemically infected leaves in a plant infected with TMV-GFP. (F) GFP fluorescence is restricted to inoculated leaves in the plant coinoculated with TMV-GFP and PVX-GF. (G and H) Coinoculation with TMV-GFP and either PVX-PSγb-GF or PVX-BSγb-GF, respectively, showed that hordeivirus γb expression allows systemic infection with TMV-GFP. Arrows indicate the systemically infected leaves showing GFP fluorescence.