. 2005 Jul 1;89(3):1990–2003. doi: 10.1529/biophysj.104.055798

TABLE 1.

Kinetic parameters for eelAChE in the ethanol and ionic solutions

	LiCl
Ic (M)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m(M⁻¹ s⁻¹ × 10⁻⁷)
0.0154	745.4 ± 13.2	14.71 ± 0.09	5.07 ± 0.06
0.0254	876.6 ± 23.6	24.13 ± 0.61	3.63 ± 0.01
0.0394	678.7 ± 0.01	24.89 ± 0.40	2.73 ± 0.04
0.0794	830.6 ± 9.5	44.91 ± 0.75	1.85 ± 0.05
0.1594	869.4 ± 5.8	70.46 ± 2.90	1.23 ± 0.06
0.3114	711.1 ± 1.9	87.88 ± 3.06	0.81 ± 0.03
	NaCl
Ic (M)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m(M⁻¹ s⁻¹ × 10⁻⁷)
0.0154	743.1 ± 11.0	14.97 ± 0.13	4.96 ± 0.12
0.0254	880.6 ± 11.8	23.20 ± 0.09	3.80 ± 0.07
0.0394	888.1 ± 0.02	26.73 ± 0.04	3.32 ± 0.01
0.0794	880.0 ± 25.2	42.13 ± 1.67	1.96 ± 0.02
0.1594	993.1 ± 16.8	83.99 ± 3.96	1.17 ± 0.08
0.3114	968.6 ± 6.5	117.17 ± 7.15	0.83 ± 0.06
	KCl
Ic (M)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m(M⁻¹ s⁻¹ × 10⁻⁷)
0.0154	773.9 ± 20.4	18.12 ± 0.33	4.27 ± 0.05
0.0254	876.6 ± 7.5	23.07 ± 0.54	3.37 ± 0.17
0.0394	920.9 ± 8.9	41.41 ± 1.06	2.22 ± 0.11
0.0794	967.8 ± 9.9	63.61 ± 1.44	1.52 ± 0.07
0.1594	813.9 ± 2.7	84.78 ± 2.82	0.96 ± 0.05
0.3114	920.6 ± 12.7	131.64 ± 0.76	0.70 ± 0.02
	RbCl
Ic (M)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m(M⁻¹ s⁻¹ × 10⁻⁷)
0.0154	839.9 ± 0.1	18.26 ± 0.75	4.60 ± 0.19
0.0254	774.6 ± 5.9	24.88 ± 1.59	3.12 ± 0.22
0.0394	762.1 ± 12.1	28.51 ± 0.43	2.63 ± 0.07
0.0794	821.8 ± 2.4	70.23 ± 1.97	1.17 ± 0.04
0.1594	922.8 ± 5.0	101.83 ± 7.65	0.91 ± 0.07
0.3114	874.0 ± 1. 8	129.05 ± 2.29	0.68 ± 0.01
	CsCl
Ic (M)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m(M⁻¹ s⁻¹ × 10⁻⁷)
0.0154	786.6 ± 0.1	19.03 ± 1.03	4.13 ± 0.22
0.0254	874.3 ± 6.8	29.61 ± 1.30	2.95 ± 0.10
0.0394	686.2 ± 4.7	33.00 ± 0.39	2.08 ± 0.04
0.0794	879.2 ± 14.0	90.72 ± 1.43	0.97 ± 0.03
0.1594	941.6 ± 35.4	168.94 ± 5.98	0.56 ± 0.01
0.3114	730.5 ± 8.9	187.23 ± 4.83	0.39 ± 0.01
	KBr
Ic (M)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m(M⁻¹ s⁻¹ × 10⁻⁷)
0.0154	861.5 ± 25.8	19.46 ± 0.34	4.43 ± 0.08
0.0254	897.6 ± 8.1	21.74 ± 0.15	4.13 ± 0.06
0.0394	889.4 ± 3.9	42.83 ± 0.70	2.08 ± 0.04
0.0794	898.6 ± 0.9	59.20 ± 0.67	1.52 ± 0.02
0.1594	996.9 ± 4.1	113.20 ± 1.80	0.88 ± 0.02
0.3114	882.3 ± 14.4	129.73 ± 1.75	0.68 ± 0.02
	KI
Ic (M)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m(M⁻¹ s⁻¹ × 10⁻⁷)
0.0154	710.9 ± 24.0	15.86 ± 0.75	4.48 ± 0.06
0.0254	748.6 ± 10. 5	25.79 ± 0.22	2.90 ± 0.02
0.0394	750.3 ± 4.9	34.80 ± 0.26	2.16 ± 0.02
0.0794	804.4 ± 4.7	64.44 ± 1.12	1.25 ± 0.03
0.1594	756.7 ± 0.7	88.72 ± 1.90	0.85 ± 0.02
0.3114	612.0 ± 7.8	120.32 ± 1.51	0.51 ± 0.01
	CsI
Ic (M)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m(M⁻¹ s⁻¹ × 10⁻⁷)
0.0154	587.3 ± 2.9	24.95 ± 0.51	2.35 ± 0.06
0.0254	639.9 ± 4.2	42.86 ± 1.23	1.49 ± 0.05
0.0394	651.4 ± 2.4	61.12 ± 1.92	1.07 ± 0.03
0.0794	630.4 ± 8.1	125.64 ± 0.48	0.50 ± 0.01
0.1594	446.9 ± 2.5	170.44 ± 9.67	0.26 ± 0.02
0.3114	274.4 ± 0.2	324.69 ± 2.41	0.08 ± 0.001
	EtOH
Ic (M)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m(M⁻¹ s⁻¹ × 10⁻⁷)
0.1	915.6 ± 2.1	18.32 ± 0.28	5.00 ± 0.07
0.2	733.5 ± 7.5	20.41 ± 0.04	3.60 ± 0.04
0.3	799.8 ± 4.6	24.74 ± 0.08	3.23 ± 0.03
0.4	718.8 ± 6.2	27.25 ± 0.07	2.64 ± 0.03
0.5	786.7 ± 0.1	28.93 ± 0.51	2.72 ± 0.05
0.6	781.4 ± 3.1	28.97 ± 0.11	2.70 ± 0.02