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. 2005 Jul 22;89(3):2134–2145. doi: 10.1529/biophysj.105.060160

FIGURE 4.

FIGURE 4

Differences between movement characteristics of the two VirE2-ssDNA complexes. Panels A–C are the probability distribution function histograms of the protein-DNA complex movements in the extract, for time interval of 20 s (see Materials and Methods). (A) plVirE2 complex in an untreated sample (6593 points from 20 traces). (B) anVirE2 complex in an untreated sample (18,359 points from 11 traces). (C) anVirE2 complex in a sample treated with nocodazole to depolymerize microtubules (3631 points from 15 traces). Curves are fits to the Gaussian PDF as described in the text, using a quasidiffusion coefficient D as the fitting parameter. (D) Mean square displacements (MSD) of the same experiments in panels A (□), B (•), and C (▪). Lines are best fits to the data, using the same dash schemes as above. Fitting expressions are also shown, where 〈x2〉 ∼ tγ. The quasidiffusion coefficient relates to the ordinary diffusion coefficient via a factor of t1−γ.