TABLE 2.
Comparison of TRS efficiencies in MHV recombinants with genomic rearrangements
| Virus | Genomea | Molar fraction of sgRNAb
|
|||||
|---|---|---|---|---|---|---|---|
| 2a/HE | S | 4 | 5a/E | M | N | ||
| MHV-WT | 1a/1b-2a/HE-S-4-5a/E-M-N | 0.005 | 0.015 | 0.138 | 0.059 | 0.160 | 0.623 |
| MHV-SmNEM | 1a/1b-2a/HE-S/m-N/[4]-5a/E-M | 0.007 | 0.017 | 0.125 (P-3) | 0.293 (P-3) | 0.559 (P-5) | |
| MHV-Δ2aHE | 1a/1b-S-4-5a/E-M-N | 0.009 (C) | 0.155 | 0.060 | 0.174 | 0.602 | |
| MHV-EMSmN | 1a/1b-4-5a/E-M-S/m-N | 0.026 (C, P-3) | 0.137 (C, P-5) | 0.046 (P-5) | 0.114 (P-5) | 0.678 | |
| MHV-MSEN | 1a/1b-M-S-4-5a/E-N | 0.014 (C, P-3) | 0.239 (P-3) | 0.103 (P-3) | 0.063 (C, P-5) | 0.581 (C) | |
a
m, 126-nt segment from the 3′ end of the M gene; [4], 106-nt segment from the 3′ end of gene 4. Each figure or capital letter represents both the gene and its natural TRS.
b
Data are the same as in Table 1. Each value is the mean from three experiments. Standard deviations have been omitted to facilitate comparison. Each sgRNA is indicated by its most upstream gene and its respective TRS. C, upstream context of the TRS is changed with respect to the wild type; P-5, genomic position of the TRS is changed in the upstream direction with respect to the wild type; P-3, genomic position of the TRS is changed in the downstream direction with respect to the wild type.