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. 2005 Jul 29;89(4):2286–2295. doi: 10.1529/biophysj.104.054080

FIGURE 3.

FIGURE 3

Interplay of uptake and activity dynamics. (A) Predicted difference in cytoplasmic concentration of a fast binding (ka = 10.0 μM−1 min−1) versus nonbinding (ka = 0) oligonucleotide. Base case parameters were used in the model except for a cytoplasmic degradation rate of 0.003 min−1. (B) Experimental results for uptake of fluorescently labeled oligonucleotides that are complementary (A-ODN157) or noncomplementary (A-ODNcontrol) as a function of time after delivery to CHO-pd1EGFP cells, as determined by flow cytometry. (C) Predicted dynamics of antisense activity for the same parameter set as in A. (D) Experimentally determined geometric mean pd1EGFP fluorescence as determined by flow cytometry for treatment of the same samples analyzed in B for the complementary oligonucleotide A-ODN157. Experimental values shown are mean ± SE, with the error bars in D being mostly smaller than the symbols.