TABLE 1.
Values of model parameters
| Parameter | Meaning | Base value | References |
|---|---|---|---|
| k1 | Adsorption of antisense to receptor (saturable) | 0.15 μM−1 min−1 | (10,12) |
| k−1 | Desorption of antisense from receptor | 0.03 min−1 | (11) |
| ki | Internalization | 0.03 min−1 | (11) |
| kr | Recycling of receptors | 0.06 min−1 | (42) |
| kt | Trafficking to mRNA locale (cytoplasm) | 0.003 min−1 | (11) |
| ka | Antisense-mRNA association (target) | 0.1–10 μM−1 min−1 | (17) |
| kd | Antisense-mRNA dissociation (target) | 0.001 min−1 | (17) |
 |
Antisense-nontarget association | 10−3μM−1 min−1 | Estimated |
 |
Antisense-nontarget dissociation | 0.001 min−1 | (17) |
| veff | RNase H cleavage | 0.003 min−1 | (17) |
| σm | mRNA synthesis rate | 15 cell−1 min−1 | (20) |
| δm | Natural decay of target mRNA | 0.03 min−1 | (20,28) |
| δb | Decay of antisense in bulk | 0.003 min−1 | (11,16) |
| δi | Degradation or efflux of internalized antisense | 0.06 min−1 | (11,43) |
| δc | Decay of antisense in cytoplasm | 0.12 min−1 | (11,16) |
| Ab0 | Initial bulk concentration | 600 nM | Chosen |
| R0 | Free receptors on surface | 1 × 105 cell−1 | (10,12,44) |
| M0 | Target mRNA | 500 cell−1 | (20,28) |
| N0 | Nontarget mRNA | 7 × 106 cell−1 | (20) |
| φ | Extracellular phase ratio | 5 × 10−9 cell μM molecule−1 | Estimated |