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(a) Schematic drawing of TWJ with a single bulge. (b) Schematic drawing of TWJ with a single branch. (c) Representative gel (12.5%), from lanes 1–4 showing mutated TWJ with 4 bp bulge, mutated TWJ with 2 bp branch, wild-type TWJ with 4 bp bulge, and wild-type TWJ with 2 bp branch.