FIG. 10.

Comparison of RNA synthesis by Δ51 and Δ21. (A) Effects of increasing concentration of Mg²⁺ or Mn²⁺ using 80 nM Δ51 and 125 nM template LE21. (B) Effects of increasing concentrations of Mn²⁺ on repressing primer extension by an 80 nM concentration of either Δ51 or Δ21 in the presence of 4 mM Mg²⁺. Lengths of the RdRp products, in nucleotides, are on the sides of the autoradiogram. Quantifications of the products synthesized by de novo initiation (the 21-mer) and primer extension (the 34-mer) are shown below the autoradiogram. The product in the lane with the number 100 in boldface type was used to normalize other products in the autoradiogram.