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Mn2+ decreases the Km for GTP. The Km for GTP as the initiation substrate nucleotide was determined using a Lineweaver-Burke double-reciprocal plot. The Km is identified from the x intercept. (A) Km titration performed in the presence of 4 mM Mg2+ and 1 mM Mn2+. (B) Km titration performed in the presence of 4 mM Mg2+.
