FIG. 8.

The effects of Mn²⁺ on primer extension by the HCV Δ21 from template LE21. Conditions used in each reaction are indicated above the autoradiogram. The de novo-initiated RNA is 21 nt, and the primer extension product is 34 nt. The 42-nt RNA is generated by the HCV ternary complex that did not terminate synthesis from LE21 but used a second molecule of LE21 to continue synthesis. This RNA is initiated de novo and is a recombination product. Quantifications of the products synthesized by de novo initiation (the 21-mer) and primer extension (the 34-mer) are shown below the autoradiogram. The product in the lane with the number 100 in boldface type was used to normalize other products.