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. 2002 Dec;76(24):12526–12536. doi: 10.1128/JVI.76.24.12526-12536.2002

FIG. 2.

FIG. 2.

Specific recognition of the initiation cytidylate by plant viral replicases and by recombinant viral RdRps. (A) Sequence and RNA secondary structure of the template B2(-)26g and its chemically synthesized variants that contain specific modifications. The modified nucleotides are numbered according to their position as template nucleotides. The secondary structure of B2(-)26g was established by nuclear magnetic resonance analysis (39). The two bases denote a normal cytosine and a 4SU. The methyl group attached to the C-5 position in T1CH3 is shown in parentheses. (B) RNA synthesis by the BMV replicase using either B2(-)26g or the modified variants of B2(-)26g listed on top of the autoradiogram. (C) RNA synthesis by the CMV replicase. (D to F) RNA synthesis by the HCV Δ21, the BVDV Δ23, and the GBV Δ23 RdRps using the templates listed above the autoradiogram.