TABLE 3.

Effect of GTP analogs on the K_m of RNA synthesis^a

Analog	Mean Km ± SD (μM)
	HCV Δ21		BVDV Δ23		GBV Δ23
	+Mn2+	−Mn2+	+Mn2+	−Mn2+	+Mn2+	−Mn2+
GTP	3 ± 0.3	103 ± 12	24 ± 5	168 ± 16	1 ± 0.2	28 ± 5
dGTP	48 ± 11		87 ± 29	103 ± 28
GDP	16 ± 3		54 ± 3
GMP	9 ± 2	434 ± 70	31 ± 6	512 ± 44

^aAll reactions contained 4 mM Mg²⁺. Where indicated (+Mn²⁺), Mn²⁺ was added to a final concentration of 1 mM. LE19 was the template used for the K_m determinations. −Mn²⁺, no Mn²⁺ added.