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Composition of fusion proteins
| Fusion name | Composition (fluorophore-linker-fluorophore) | Linker length (aa) | Protease sensitivity | Linker type (aa motifs) |
|---|---|---|---|---|
| fCasp3,s | EGFP-linker23-mRFP1 | 23 | Casp-3 | S-G |
| fCasp3,l | RsGFP-linker48-tdimer2(12) | 48 | Casp-3 | A-G/S-G |
| finert,s | EGFP-inert23-tdimer2(12) | 23 | inert | A-G/S-G |
The names of fusion constructs indicate the design of their protein linkers according to proteolytic sensitivity (inert; Casp-3) and linker length (short, 23 aa; long, 48 aa).
