TABLE 3.

Measurement parameters of intracellular dcFCCS analysis

Fusion	Time/figure	CCrel (%)	Molecular brightness η of GFP (kHz)	Molecular brightness η of RFP (kHz)	c(GFP) (nM)	c(RFP) (nM)	c(fusion) (nM)
EGFP and tdimer2(12)	Fig. 4 A	11 (±2)	1.7 ± 0.1	1.4 ± 0.1	12	18	(neg. control)
finert,s	Fig. 4 B	50 (±3)	1.0 ± 0.01	1.8 ± 0.02	137	20	10 ± 2
finert,s	Fig. 7, cell 1
	0 h	47 (±1)	1.5 ± 0.02	1.3 ± 0.04	353	41	19 ± 2
	5 h	46.5 (±1.5)	0.8 ± 0.01	1.1 ± 0.05	185	58	27 ± 3
	23h	43 (±5)	0.8 ± 0.02	1.3 ± 0.02	112	105	46 ± 9
fCasp3,s	Fig. 7, cell 2
	0 h	44 (±1)	1.2 ± 0.02	2.0 ± 0.05	129	31	13 ± 2
	5 h	30 (±2)	0.6 ± 0.01	0.7 ± 0.1	85	29	9 ± 2
fCasp3,l	Fig. 7, cell 3
	0 h	34 (±1)	0.8 ± 0.02	0.8 ± 0.01	291	157	53 ± 2
	21 h	18 (±5)	0.7 ± 0.01	0.5 ± 0.01	88	56	10 ± 3

All measurements presented in this study are summarized and characterized in this table. The figures and cells corresponding to each row of data are indicated in column 2. Relative cross correlation is presented along with molecular brightness values. The molecular brightness η corresponds to the average photon count per molecule and was obtained according to Eq. 3, whereas fit errors were considered for error estimates. Moreover, intracellular concentrations of all fluorescent specimens have been calculated. The concentration of fluorophores was determined with an estimated maximum error of ±10%, including fit errors of calibration and intracellular measurements, deviations among different fit models, and chromatic effects within cells.