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. 2005 Jul 29;89(4):2855–2864. doi: 10.1529/biophysj.105.062554

FIGURE 1.

FIGURE 1

MPA of isolated nuclei shows power-law creep behavior dominated by the DNA. (A) An isolated nucleus aspirated into a micropipette of 6 μm inner diameter at a constant applied pressure of 15 kPa shows deformation of the nucleus as well as the nucleoli (arrowheads). (B) Creep compliance, J, as a function of time shows power-law behavior over four decades with an exponent α ≈ 0.3. When the pressure is released, the projection length decreases with the same exponent. (C) Aspirated nuclei, with GFP-lamin B and Hoechst 33342-labeled DNA, show the relative contributions under strain. The DNA appears to percolate throughout the nucleus and fill the micropipette, and lamins appear to be dominated by an internal network which causes buckling at sites exterior to the micropipette.