TABLE 1.

Relative hybridization to two immobilized spots

	Rev. rate const. kr1	Initial molar amounts of			% Hybridized			Expected signal on		Time to equilibrium
Row		Immob. A (correct)	Immob. B (incorrect)	C (sol.)	A	B	C	A (correct)	B (incorrect)
1	0.01	10	1	1	9.8	1.1	99.9	0.98	0.01	long
2	0.01	1	1	1	74.5	22.6	97.1	0.745	0.226	long
3	0.01	1	1	10	99.9	98.8	19.9	1	0.99	short
4	0.01	1	10	1	39.1	6	99.4	0.39	0.6	long
5	0.01	1	10	10	98.2	84.7	94.5	0.98	8.47	short
6	0.001	10	1	1	10	0.1	100	1	0.001	long
7	0.001	1	1	1	90.4	8.6	99.1	0.9	0.086	long
8	0.001	1	1	10	100	98.8	19.9	1	0.988	short
9	0.001	1	10	1	73.2	2.7	99.7	0.73	0.27	long
10	0.001	1	10	10	99.8	84.5	94.5	0.99	8.45	short

The rate constants for these simulations were k_f1 = k_f2 = 1; k_r2 = 0.1. Short equilibrium times denote values of ≤5, while long times are >50.