TABLE 2.

Hybridization washing results

		Initial molar amounts of			Expected signal before washing		Expected signal after long washing		Expect. signal ratio after washing
Row	Rev. rate const. kr1	Immob. A (correct)	Immob. B (incorrect)	C (sol.)	A (correct)	B (incorrect)	A (correct)	B (incorrect)	for opt. time	at t = 1000	Opt. wash time
1	0.01	10	1	1	0.98	0.01	0.883	0.0096	144.4	92	9
2	0.01	1	1	1	0.745	0.226	0.359	0.053	15.03	6.77	32
3	0.01	1	1	10	1	0.99	0.567	0.116	6.66	4.9	40
4	0.01	1	10	1	0.39	0.6	0.269	0.354	1.1	0.758	17
5	0.01	1	10	10	0.98	8.47	0.839	3.42	0.251	0.245	24
6	0.001	10	1	1	1	0.001	0.99	0.001	1526	902	9
7	0.001	1	1	1	0.9	0.086	0.714	0.024	79.7	29.5	43
8	0.001	1	1	10	1	0.988	0.908	0.09	10.99	10.09	60
9	0.001	1	10	1	0.73	0.27	0.644	0.176	5.17	3.65	24
10	0.001	1	10	10	0.99	8.45	0.981	3.372	0.291	0.291	45

The rate constants for these simulations were k_f1 = k_f2 = 1; k_r2 = 0.1. The washing dilution factor was set at 100. All washings were done for a time of 1000. By this time, CB had come to a virtual equilibrium, but CA generally was still decreasing.