TABLE 3.

Acrylamide quenching of gramicidin in SDS micelles

Host*	KSV† (M−1)	kq (× 10−9)‡ (M−1s−1)
SDS micelles	8.46 ± 0.51	5.79
SDS micelles (in the presence of 0.5 M NaCl)	2.20 ± 0.07	2.42

^*The ratio of gramicidin/SDS was 1:4000 (mol/mol). The excitation wavelength was 295 nm; emission was monitored at 334 nm. See Materials and Methods for other details.

^†Calculated using Eq. 3. The quenching parameter shown represents the means ± SE of three independent measurements whereas quenching data shown in Fig. 7 are from representative experiments.

^‡Calculated using mean fluorescence lifetimes from Table 2 and using Eq. 3.