TABLE 1.
Gating rate and equilibrium constants
| Construct | Opening rate constant (s−1) | Closing rate constant (s−1) | Gating equilibrium constant (Θ) |
|---|---|---|---|
| δ268S | 120 | 2170 | 0.055 |
| δ268G | 131 | 967 | 0.136 |
| δ268C | 143 | 200 | 0.715 |
| δ268T | 164 | 190 | 0.863 |
| δ268I | 177 | 143 | 1.23 |
| δ268N | 228 | 45.8 | 4.98 |
| δ268S + αT422 | 11.0 | 7020 | 1.57 E−3 |
| δ268G + αT422 | 13.9 | 3600 | 3.86 E−3 |
| δ268C + αT422 | 28.0 | 1373 | 0.020 |
| δ268T + αT422 | 20.9 | 846 | 0.025 |
| δ268I + αT422 | 27.2 | 770 | 0.035 |
| δ268N + αT422 | 46.2 | 325 | 0.142 |
| δ268S + ɛP121L | 0.75 | 4780 | 1.57 E−4 |
| δ268C + ɛP121L | 3.60 | 2530 | 1.42 E−3 |
| δ268T + ɛP121L | 5.00 | 2450 | 2.04 E−3 |
| δ268I + ɛP121L | 8.10 | 2300 | 3.52 E−3 |
| δ268N + ɛP121L | 26.4 | 1850 | 0.014 |
| δ268S + αT422+ ɛP121L | 0.03 | 9890 | 3.03 E−6 |
| δ268G + αT422+ ɛP121L | 0.05 | 6880 | 7.26 E−6 |
| δ268C + αT422+ ɛP121L | 0.25 | 6090 | 4.11 E−5 |
| δ268T + αT422+ ɛP121L | 0.28 | 5570 | 5.03 E−5 |
| δ268I + αT422+ ɛP121L | 0.38 | 5480 | 6.93 E−5 |
| δ268N + αT422+ ɛP121L | 1.18 | 4140 | 2.85 E−4 |