FIGURE 3.

Histogram analysis of single molecule fluorescence assays. The distances traveled in individual events of processive movement along the MTs were combined in a histogram. The data were fit by a single exponential, the time constant of which represents the average run length. Panel A shows data for NcKin on MTs (data from Lakamper et al. (24); N = 182; run length 1.75 ± 0.09 μm). Panel B shows the histogram for NcKin motors moving along subtilisin-treated MTs (N = 67; run length 0.89 ± 0.08 μm, Table 1).