TABLE 2.
Summary of kinetic data for native and digested microtubules
 |
 |
 |
 |
 |
 |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NK343
|
NK378
|
NK383
|
NK391
|
NK400
|
NK433
|
|||||||
| Motor | MT | dMT | MT | dMT | MT | dMT | MT | dMT | MT | dMT | MT | dMT |
| kcat [ATP/head*s] | 247.6 ± 22.2 (7) | 249.4 ± 17.0 (6) | 18.81 ± 1.69 (12) | 33.64 ± 2.89 (10) | 20.53 ± 2.71 (10) | 32.92 ± 5.56 (8) | 71.72 ± 5.16 (8) | 50.01 ± 4.10 (8) | 68.59 ± 7.35 (7) | 49.84 ± 6.00 (7) | 64.18 ± 4.45 (13) | 46.10 ± 4.16 (11) |
| k0.5,MT [μM] | 0.94 ± 0.35 (6) | 0.76 ± 0.21 (5) | 7.44 ± 1.21 (12) | 13.13 ± 1.54 (10) | 6.76 ± 1.50 (10) | 11.72 ± 3.14 (8) | 0.21 ± 0.05 (5) | 0.31 ± 0.08 (5) | 0.11 ± 0.01 (6) | 0.16 ± 0.05 (6) | 0.23 ± 0.06 (13) | 0.46 ± 0.13 (10) |
| kbi,ADP [μM−1s−1] | 19.6 ± 0.94 (13) | 24.75 ± 2.78 (12) | 2.58 ± 0.30 (4) | 2.05 ± 0.08 (4) | 3.44 ± 0.13 (4) | 3.18 ± 0.13 (4) | 14.16 ± 1.02 (4) | 14.99 ± 0.72 (4) | 20.44 ± 1.55 (4) | 14.12 ± 0.65 (4) | 16.57 ± 1.47 (7) | 9.77 ± 0.99 (7) |
| kbi, ratio [#]* | 13.45 | 13.26 | 0.98 | 1.24 | 0.88 | 0.88 | 24.11 | 10.76 | 30.51 | 22.06 | 16.84 | 10.26 |
All errors given as ± se, number of independent assays given in parenthesis.
*
kbi,ratio,MT is defined as (kcat,MT/ K0.5, MT) / (kbi,ADP, MT), representing the number of ATP hydrolyzed per productive diffusional encounter of motor with MT.