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. 2005 Aug 19;89(5):3345–3352. doi: 10.1529/biophysj.105.068759

FIGURE 1.

FIGURE 1

(A) Model of prestin's transmembrane topology that posits 10 transmembrane domains with intracellular amino and carboxy termini of the protein. Shown are the positions (red circles) of individual truncations of the protein that we tested. A cartoon of the crystal structure of the C-terminus of prestin modeled on the bacterial protein SpoIIAA, to which it shows strong homology, is shown. The positions of two peptides that would lie on the extracellular surface of the protein between transmembrane domains 4 and 5 (peptide A) and 6 and 7 (peptide B) are indicated. (B) Affinity-purified antibodies against these peptides (ApA and ApB) were used to stain both live guinea pig OHC (upper panel of confocal images) and live CHO cells transfected with prestin (lower panel). These results, together with other data (see text), confirm that these antigens lie on the extracellular surface, and support the 10-transmembrane model.