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Correlation functions of a mixture of freely diffusing, noninteracting dyes: Atto532 (2.5 nM) and Atto647 (2.5 nM). The CCF with (black) and without (gray) the elimination of cross talk are shown, normalized to the ACF of Atto647 (light gray). An amplitude of 13.0% is observed for the CCF without PIE, whereas the cross-correlation amplitude with PIE shows an amplitude of −0.7%.
