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SiRNAs directed against tat, RT, and p65 inhibit HIV-1 replication in Jurkat cells. Jurkat cells (n = 2 × 105) were transfected with Oligofectamine containing 100 pmol of siRNAs corresponding to either tat1, tat2, and tat3; p65 and RT1 and -2; Tax or mock transfected. After 16 h, the cells were washed, resuspended in RPMI medium, and infected with HIV-1 NL4.3, and p24 antigen levels were determined by ELISA at 3 days postinfection.
