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. 2005 Aug 19;89(5):3615–3627. doi: 10.1529/biophysj.105.059295

FIGURE 1.

FIGURE 1

FCS measures vesicle dynamics in single synapses. (A) A schematic of the FCS detection volume (light box) is shown overlying a typical large synapse labeled with FM 1–43 (scale bar 0.5 μm). (B) A plot of the normalized intensity measured by scanning the detection volume along the long (▵) and the short (▪) axes of this synapse as well as along a 40 nm fluorescent bead (○). A plot of the Gaussian light box I = I0exp(−r2/2w2) (solid line), where w = 0.11 μm, is also shown. (C) The intensity trace measured from a single synapse is fit to an exponential function (shaded trace) and is used to calculate the autocorrelation function G(τ) shown in (D) (see Materials and Methods).