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. 2005 Aug 19;89(5):3615–3627. doi: 10.1529/biophysj.105.059295

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Copyright © 2005, Biophysical Society

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Synapse center of mass motion does not contribute to the FCS measurement. (A) The inset displays the trajectory of the center of mass of a typical synapse (0.3 s/step, totaling T = 90 s, scale 5 nm). The mean squared displacement calculated from this synapse's trajectory is plotted below the inset. (B) The average was calculated for 30 synapses and is displayed (top line with error bars) along with a linear fit (shaded line) of slope The average mean-square displacement for 0.42 μm fluorescent beads (bottom solid line, with the thickness exceeding the error, n = 75) adsorbed to a glass coverslip is displayed along with a linear fit (shaded line) of slope 4D_CM = 0.22 nm²/s.

Inline graphic — Synapse center of mass motion does not contribute to the FCS measurement. (A) The inset displays the trajectory of the center of mass of a typical synapse (0.3 s/step, totaling T = 90 s, scale 5 nm). The mean squared displacement calculated from this synapse's trajectory is plotted below the inset. (B) The average was calculated for 30 synapses and is displayed (top line with error bars) along with a linear fit (shaded line) of slope The average mean-square displacement for 0.42 μm fluorescent beads (bottom solid line, with the thickness exceeding the error, n = 75) adsorbed to a glass coverslip is displayed along with a linear fit (shaded line) of slope 4D_CM = 0.22 nm²/s.