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. 2005 Aug 19;89(5):3615–3627. doi: 10.1529/biophysj.105.059295

FIGURE 8.

FIGURE 8

The total fluorescence of FM 1–43 labeled synapses. Image of a field of FM 1–43 labeled puncta before (A) and after (B) unloading with three rounds of high K+ bath solution. (C) The difference image of B subtracted from A. Identical ROIs were drawn around puncta in A and B, and the total intensity was calculated for each ROI. (D) A histogram of the starting intensities for three experiments is displayed in counts on the CCD camera per second. (E) A histogram of the difference intensities for the same experiments.