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. 2002 Dec;76(24):12908–12916. doi: 10.1128/JVI.76.24.12908-12916.2002

FIG. 3.

FIG. 3.

A CMV RNA1 transgene does not enhance the replication of TMV-GFP in NN tobacco. The upper panel shows Northern blot hybridization analysis of the accumulation of viral RNA in protoplasts isolated from tobacco cultivar Samsum NN, either not transformed or transgenic for CMV RNA1 (labeled NN and NN-TG, respectively), 24 h after electroporation with water (lane M), WT TMV transcript RNA (lane 1), TMV-GFP transcript RNA (lane 2), PVX transcript RNA (lane 3), or PVX-GFP transcript RNA (lane 4). The lower panel shows rRNA accumulation.