Extended Data Fig. 2. Surface electrostatics of carboxylic acid-functionalized PS microparticles.

a, A diagram illustrating different surface electrostatics of carboxylic acid functionalized PS microplastics in different solvent environments. b, Zeta potential of carboxylic acid functionalized PS microplastics in 1 mM NaCl containing 0%, 0.001%, 0.01% or 0.1% Triton X-100 (n = 5 independent measurements per concentration). c, Zeta potential of carboxylic acid functionalized PS microplastics in water (n = 10 independent measurements) and ethanol (n = 4 independent measurements). Bars show the mean ± s.d. d-f. Fluorescence imaging of Rh800 localized on the surface of carboxylic acid functionalized PS microplastics (d) in water, (e) with Triton X-100, and (f) in ethanol environment. The negative surface charge of PS microplastics arising from oxygen-containing functional groups, such as carboxylic acids, shows distinct insensitivity to solvent conditions compared to the negative charges originating from water-hydrophobe interactions.