FIG. 2.
Effects of pH, salt, and temperature on E. coli O157:H7 viability and peptide structure. (A to C) Dose responses obtained after 2 h of incubation with P28 (A), P14 (B), and NC12-P14 (C). Symbols and peptide concentrations are as specified for Fig. 1. CFU values represent the means ± standard deviations obtained from two independent experiments performed in duplicates. Zero CFU indicates negative cultures. (D) Effect of incubation conditions on NC12-P14 secondary structure. Circular dichroism spectra were measured for peptide samples (100 μM) in buffer containing liposomes (2 mM PC-PG [3:1]). Data represent average values from two separate experiments.