FIG. 1.

TMEV-induced cytotoxic responses against various cell lines. Three weeks after TMEV infection, spleen cells were isolated from SJL/J mice, incubated with TMEV-APCs for 6 days, and used as effector cells in a ⁵¹Cr release assay. As target cells, three mouse fibroblast cell lines were used: syngeneic PSJL (H2^s) (○), BxSF1 (H2^b/s) (▵), and allogeneic target C57SV (H2^b) (•). PSJL and C57SV cells were derived from SJL/J mice and C57BL mice, respectively. BxSF1 cells were derived from F₁ mice resulting from a cross between SJL/J and C57BL mice. We detected high and moderate autoreactive cytotoxic responses against PSJL and BxSF1 cells but much lower cytotoxicity against C57SV cells. An astrocyte cell line (□) was also used as a target and was killed the least. Results are representative of those from 10 independent experiments. Error bars indicate standard errors.