FIG. 2.
Cytotoxicity against target cells that express TMEV antigens. In a 51Cr release assay, we used TMEV-induced autoreactive cells as effector cells. As target cells, we used uninfected PSJL cells (•), PSJL cells pulsed with TMEV at an MOI of 10 (DAs-PSJL) (▵), PSJL cells infected with DA virus at an MOI of 5 for 18 to 24 h (DAL-PSJL) (□), and PSJL cell lines transfected with TMEV capsid proteins VP1, VP2, VP3, and VP4 (PSJL-VP1 [▿], PSJL-VP2 [×], PSJL-VP3 [+], and PSJL-VP4 [○], respectively). Although TMEV-infected targets showed slightly higher killing, uninfected PSJL cells showed killing similar to that of other cell lines. Results are representative of those from four independent experiments.