FIG. 4.
Effect of ACHP on chronically HIV-1 infected cells. (A and B, left) Effect of ACHP on HIV-1 production. MOLT4/IIIB cells (2 × 105/ml) were pretreated with or without ACHP for 1 hour and then stimulated with or without TNF-α for 24 h. Cell supernatants were then collected and analyzed for HIV-1 p24 antigen levels by ELISA. (A and B, right) Cytotoxicity of ACHP on MOLT4/IIIB cells. Cell viability was determined by the WST-1 assay. The CC50 value was extrapolated from this measurement. The data are mean values plus standard deviations of triplicate experiments.