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Effect of K+-glutamate on ciprofloxacin-mediated DNA cleavage by E. coli wild-type and chimera 7 gyrases. The ciprofloxacin concentration is indicated. N, L, and S, nicked, linear, and supercoiled DNA, respectively. The K+-glutamate concentration in the reactions were (top) 0 mM, (middle) 100 mM, and (bottom) 300 mM.
