TABLE 3.

Ciprofloxacin inhibition of gyrase DNA supercoiling activity and stimulation of cleavage complex formation with gyrase

Recombinant enzyme	IC50 (μg/ml) with the following K+-glutamate concn:			CC50 (μg/ml) with the following K+-glutamate concn:
	0 mM	100 mM	300 mM	0 mM	100 mM	300 mM
E. coli GyrA2 GyrB2	0.16-0.31	0.62-1.25	0.62-1.25	0.31	0.16-0.31	0.62
Chimera 7 GyrA2 and E. coli GyrB2	0.62-1.25	0.16-0.31	0.62-1.25	0.62-1.25	1.25	5