TABLE 4.

Activities of ciprofloxacin against KNK453 (gyrA^ts) transformed with pBAD24-gyrase genes on different agar media^a

Plasmid	Ciprofloxacin MIC (μg/ml) in the following growth mediab:
	I	II	III	IV
KL16 gyrAc	0.02	0.04	0.04	0.06
S83Ld	0.08	0.08-0.16	0.08-0.16	0.08-0.16
Chimera 7e	0.02	0.08	0.04	0.06

^aDetermined by agar dilution method at 43°C.

^bI, minimal medium; II, medium I plus K⁺-glutamate 300 mM; III, minimal medium with Casamino Acid supplementation; IV, medium III plus K⁺-glutamate at 300 mM.

^cWild-type E. coli gyrA.

^dKL16 Ser83Leu gyrA.

^eChimera of E. coli and S. aureus GyrA.