TABLE 3.

Characteristics of methods used to evaluate the efficacy of nonoxynol-9

Assay name	Laboratory	Cell line	Virus/mode of infection	Duration of compound incubation	Duration of assay	Efficacy readout	Comments
CD4 independent	SR	ME-180	Chronically HIV-1 SK-1-infected H9 cells, cell associated	4 h	24 h	p24	Virus and N-9 washed off after 4 h
CD4 dependent	SR	HOS-CD4-X4/R5	Chronically HIV-1 SK-1-infected H9 cells, cell associated	4 h	24 h	p24	Virus and N-9 washed off after 4 h
CFIA	CR	MT-2	HIV-1 RF, cell free	5 min	7 days	Microscopic evaluation of CPE	Preincubation of N-9 and virus; N-9 not washed off but compound effect terminated by dilutions
CAIA	CR	MT-2	Chronically HIV-1 RF-infected H9 cells, cell associated	5 min	7 days	Microscopic evaluation of CPE	Preincubation of N-9 and cell-associated virus; N-9 not washed off but compound effect terminated by dilutions
Tissue viability	GH	Cervical tissue	HIV-1 Ba-L, cell free	O/N	14 days	p24	N-9 and cells incubated 1 h, prior to addition of virus, N-9 and virus washed off after O/N incubation
CAIA	DU	HCLB cells	HIV-1 IIIB-infected SupT1 cells, cell associated	10 min	48 h	X-Gal	Incubation of infected cells with N-9 for 10 min, followed by 1:10 dilution and coculture for 2 h with HeLa-CD4-LTR-β-gal cells
CFIA	DU	HCLB cells	HIV-1 IIIB, HIV-1 Ba-L, HIV-1, 89.6, cell free	10 min	48 h	X-Gal	Incubation of cell-free virus with N-9 for 10 min, followed by 1:100 dilution and coculture for 2 h with HeLa-CD4-LTR-β-gal cells