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Measurement of yeast viability following gentamicin treatment. Yeast strains were incubated overnight with gentamicin concentrations (500 μg/ml for TLG2 and YPT6 mutants, 100 μg/ml for VPS45, VPS53, VPS54, SAC1, and NHX1 mutants, and 50 μg/ml for VPS51, VPS52, VPS16, and ZUO1 mutants) by using the same conditions as those detailed in the legend for Fig. 1. The strains were than stained with methylene blue and counted. Data are presented using a box plot.
