FIG. 2.

Mutations found in S. aureus and S. pyogenes multi- and single-passage mutants with reduced susceptibilities to retapamulin. The figures shows an alignment of various ribosomal protein L3 sequences in the regions flanking the mutations. The positions of the characterized mutations in S. aureus and S. pyogenes mutants are highlighted in a shade of gray; position 149 in E. coli and analogous positions in other sequences crucial for developing resistance are indicated with an asterisk; and positions of amino acid identity are highlighted in dark gray. Sequences: 1, parental S. aureus strain starting from amino acid position 142; 2, selected S. aureus mutant starting from position 142; 3, parental S. pyogenes strain starting from position 137; 4, selected S. pyogenes mutant starting from position 137; 5, E. coli starting from position 139; 6, Brachyspira hyodysenteriae and Brachyspira pilosicoli starting from position 139; 7, Deinococcus radiodurans starting from position 134; 8, Haloarcula marismortui starting from position 232; and 9, Saccharomyces cerevisiae starting from position 245.