TABLE 3.
PCRs for quantification of P. chabaudi chabaudi mdr1, tctp, and atp6 genes in comparison to msp1a
| Gene | Sense | Primer sequence (5′-3′) | MgCl2 concn/ primer concn |
|---|---|---|---|
| mdr | Sense | TCTCGACCAAATGTACCAATATA | 3 mM/0.8 μM |
| Antisense | GCATTAGTCATTTCTATATCATTTG | ||
| tctp | Sense | AATGATGAAGTATGCTCTGACTCA | 4.5 mM/0.8 μM |
| Antisense | CATTCCTTCTACTGCATCTTCAC | ||
| atp6 | Sense | AGGCAAGTACCTTATCATTATCC | 4.5 mM/0.8 μM |
| Antisense | GTGAAGAAGTAAAGATCCTATTG | ||
| msp1 | Sense | ACAGTAACACAAGAAGGAAC | 3.5 mM/0.4 μM |
| Antisense | GATACTTGTGTTGATGCTGG |
a
PCR amplification conditions: 40 cycles of 95°C for 600 min 95°C with a 0-min hold, cooling at 20°C/s to 63°C with a 7-min hold, heating at 20°C/s to 72°C with a 7-min hold. Heating at 20°C/s to 95°C with 0-min hold, cooling at 20°C/s at 65°C and heating at 0.2°C/s to 95°C in a continuous acquisition mode produced the melting curve.